The Center
for Medical
Genomics strongly recommends that at least 4 to 6 biologically
independent
samples be used for each condition to be examined. We do not recommend
pooling
strategies. In some circumstances, pooling is useful to get enough RNA
to use,
but we still recommend at least 4 to 6 biologically independent pools.
Amount of total
RNA needed for the GeneChip‰
•
Standard protocol:
5-10 mg total RNA at > 0.75 mg/ml
•
With consultation:
2 mg to 5 mg total RNA in < 8 ml volume (we can concentrate
samples for
additional charge)
•
Special: requires
Amplification. 0.05 mg to 1 mg total RNA. Additional charge. NOTE: Amplified
samples work,
but there is truncation of the 5Õ ends and increased
variability; consider
using a larger number of samples. CONSULT WITH CMG to discuss the
experiment.
Quality Control
measures
•
Spectrum from
210
or 220 nm to 350 nm
Detailed sample and experimental
information
should be provided before the sample is brought to the CMG.