Howard J. Edenberg, Ph.D.
Professor of Biochemistry and Molecular Biology and of Medical and Molecular Genetics
Indiana University
School of Medicine
Biochemistry and Molecular Biology
I.U.


 

BlastReport: A Perl script to facilitate the use of sequence databases for mapping and clustering

Jeanette McClintick and Howard J. Edenberg

BioTechniques 29, 1272-1276 (December 2000)

BlastReport is a Perl script that sorts through BLAST output and formats it for rapid evaluation.
We also present some suggestions for BLAST settings to make database searching more efficient .
See and download the ReadMe file for details.
There are some new notes about restricting searches to subsets of the databases in the file Update.htm

BlastReport produces 3 files:
    • BRept_Full provides a summary for each query sequence followed by the alignments that are most likely to be biologically significant.
    • BRept_Sum is a list of the regions of alignment only (Fig. 2).
    • BRept_Tab is a tab-delimited file containing query name, the name of the aligning sequence and the first region of alignment. BRept_Tab can be opened in a spreadsheet such as Excel® to produce a table of alignments that can be sorted in whatever way is most useful. Sorting by the aligning sequence makes it easy to see if that sequence contains multiple hits to query sequences, such as 2 markers or a marker and a portion of a cDNA.

Download BlastReport [Save as textfile.]

View ReadMe file.
View Update file.
 
Note: BlastReport must be customized by inserting the name of your computer and folder/subdirectory before use. We suggest you download the ReadMe document for details.

Download ReadMe as .pdf


 

Link to Perl

Link to Blastcl3


Edenberg Laboratory Home Page
Center for Medical Genomics, Indiana University School of Medicine


© Copyright 1996-2000 Howard J. Edenberg
Original: 21 August 2000 Howard Edenberg
Comments: Howard Edenberg